STAMBPL1, Recombinant, Human, aa1-436, His-Tag (AMSH-like Protease, ALMalpha, AMSH-FP, AMSH-LP, bA399O19.2)

Blue Ice

52

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 20% glycerol.

STAMBPL1 is a zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains, but doesn’t cleave 'Lys-48'-linked polyubiquitin chains. The JAMM motif is essential for the protease activity.

Source:
Recombinant protein corresponding to aa1-436 from human STAMBPL1 at N-terminal, fused to His-tag expressed in E.coli.

Molecular Weight:
~52kD (458aa)

AA Sequence:
MGSSHHHHHH SSGLVPRGSQ SHMDQPFTVN SLKKLAAMPD HTDVSLSPEE RVRALSKLGC NITISEDITP RRYFRSGVEM ERMASVYLEE GNLENAFVLY NKFITLFVEK LPNHRDYQQC AVPEKQDIMK KLKEIAFPRT DELKNDLLKK YNVEYQEYLQ SKNKYKAEIL KKLEHQRLIE AERKRIAQMR QQQLESEQFL FFEDQLKKQE LARGQMRSQQ TSGLSEQIDG SALSCFSTHQ NNSLLNVFAD QPNKSDATNY ASHSPPVNRA LTPAATLSAV QNLVVEGLRC VVLPEDLCHK FLQLAESNTV RGIETCGILC GKLTHNEFTI THVIVPKQSA GPDYCDMENV EELFNVQDQH DLLTLGWIHT HPTQTAFLSS VDLHTHCSYQ LMLPEAIAIV CSPKHKDTGI FRLTNAGMLE VSACKKKGFH PHTKEPRLFS ICKHVLVKDI KIIVLDLR

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.