NEK6, Recombinant, Human, full length, N-terminal His-Tag, Serine Threonine Kinase (NIMA-Related Kinase 6, Never in mitosis A)

Molecular Biology / MB-Protein Kinases

-70°C

25 mM Tris-HCl, pH 8.0, 100 mM sodium chloride, 0.05% Tween 20, 50% glycerol, 10 mM reduced glutathione, and 3mM DTT.

Human NEK6 (GenBank Accession No. NM_014397), (a.a. 1-313) with N-terminal HIS6 tag,

MW 40.1kD, expressed in an Baculovirus infected Sf9 cell expression system.

Specific Activity: 50 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 7 peptide substrate per minute at pH 7.4 and 30°C

Assay buffer: 50mM HEPES, pH 7.4, 3mM MgCl2, 3mM MnCl2, 1mM DTT, 3uM Na-orthovanadate, 0.5mM ATP, 4uM NEK6 substrate, 0.4ug/ml NEK6.

Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Stability: >6 months at –80°C