NFAT4, Control Peptide (NFATC4, Nuclear Factor of Activated T Cells 4)

Molecular Biology / MB-Protein Kinases

-20°C

Supplied as a lyophilized powder.

Control peptide for NFATC4 (Nuclear Factor of Activated T Cells 4) N2301-35, an antibody raised against a synthetic peptide PEGYNEVGPGYTPGEGC (linear) corresponding to an epitope of human NFATC4.

NFAT (Nuclear Factor of activated T Cells) is composed by five members: NFATC (1, 2, 3 and 4) and NFAT5. Genes encoding NFAT proteins are transcribed in almost every tissue, although the expression of individual proteins is more tissue restricted. When intracellular calcium rises, the calmodulin-dependent serine-threonine phosphatase calcineurin dephosphorylates NFAT proteins in the cytoplasm, exposing their NLS (Nuclear Localization Sequence) and inducing their translocation to the nucleus. NFATc4 protein is expressed at highest levels in the developing hippocampus at about the time of birth and responds to normal synaptic activity, which transduces Ca2+ signals mediated by NMDA receptors and L-type Ca2+ channels.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Stable for 12 months at -20°C. Reconstitute to nominal volume (see reconstitution instructions for peptides) and store at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.