5'-Nucleotidase, Recombinant, Human (CD73, 5'-nucleotidase, Ecto)

Blue Ice

59

Supplied as a liquid in 20mM Tris, pH 7.5, 120mM sodium chloride, 4mM calcium chloride, 20% glycerol.

CD73, an ecto-5'-Nucleotidase, is an ectoenzyme expressed by most cell types. The 5'-Nucleotidase activity of CD73 converts extracellular nucleoside-5'-monophosphates to nucleosides, with AMP as the preferred substrate. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. These functions include the adhesion of B-cells to follicular dendritic cells and T-cell signaling CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'-Nucleotidase activity. The native enzyme is a homodimer bound to the cell membrane through a glycosyl phophatidylinositol (GPI) anchor. This recombinant human CD73 is truncated at the C-terminus, and lacks the GPI modification site. The recombinant CD73 is therefore secreted as a monomer.

Recombinant protein corresponding to Trp27-Lys547, with a C-terminal 6-His tag from human 5'-Nucleotidase expressed in CHO cells (AAH65937).

SDS-PAGE:
61-62kD under reducing conditions

Biological Activity:
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate. The specific activity is ≥15, 000pmoles/min/ug.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.