Peroxiredoxin 3, 63-256aa, Recombinant, Human (Thioredoxin-dependent peroxide reductase, mitochondrial)

Dry Ice

21, 5

Supplied as liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol

Peroxiredoxin 3, also known as PRDX3, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 3 is specifically localized in mitochondria and believed to play important roles in the regulation of cellular redox status by serving as a primary line of defense against H2O2 produced during respiration.

Recombinant protein corresponding to aa63-256 from human Peroxiredoxin 3 protein, expressed in E. coli

Amino Acid Sequence:
MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK
PSPAASKEYF QKVNQ

SpecifIc Activity:
As Reported. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of P3352-22 with peroxide for 20 minutes at RT. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25ºC for 1 minutes.

Activity Assay:
1. Prepare a 50ul reaction mix into a suitable container: The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol.
2. Add 5ul of P3352-22 with various concentrations (0.25ug, 0.5ug) in 45ul reaction buffer.
3. Incubate at 25°C for 2 minutes.
4. Add 5ul of 5mM H2O2 as a substrate and incubate the mixture for 20 minutes.
5. Add 20ul of 26% trichloroacetic acid (TCA) to stop the reaction.
6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN)
7. Record the increase in A475nm.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.