Phosphodiesterase 4B1, Recombinant, Human, GST-Tag (PDE4B1)

Dry Ice

109

Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM NaCl, 2.4mM KCl, 18mM glutathione, 3mM DTT, 10% glycerol

Human PDE4B1 (GenBank Accession No. NM_001037341), full length with N-terminal GST tag (MW: 109kD), expressed in a Baculovirus infected Sf9 cell expression system.

Specific Activity:
Lot-specific. Sample Lot Value: 8532 pmol/min/ug.

Unit Definition:
1 unit is defined as the amount of enzyme that will convert 1 pmole of 3’, 5’-cAMP to 5’AMP per min at 37°C.

Assay Conditions:
10 mM Tris-HCl, pH 7.4, 1mM MgCl2, 0.2mM 3’, 5’-cAMP substrate and 0.2ng/ul PDE4B1 at 37ºC for 20 min. Quantitified by 5’AMP product ad releasing the phosphate group which is deteted by Malachite Green Reagent.

Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.