Phosphodiesterase 6 delta, Recombinant, Human (Phosphodiesterase 6D cGMP Specific Rod delta, PDE6 delta, PDE6D, PDED, GMP-PDE delta, Protein p17, Retinal Rod Rhodopsin Sensitive cGMP 3' 5' Cyclic Phosphodiesterase Subuni

Blue Ice

18, 4

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 100mM sodium chloride, 10% glycerol.

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (ab) that is regulated by two inhibitory γ subunits. As PDE6D does not modify the catalytic properties of PDEab, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E. coli.

Recombinant protein corresponding to aa1-150 of human PDE6D with a 6x His tagged expressed in E. coli (NP_002592).

Amino Acid Sequence:
MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH

Enzyme Activity:
Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Phosphodiesterase 6 delta, Recombinant, Human (Phosphodiesterase 6D cGMP Specific Rod delta, PDE6 delta, PDE6D, PDED, GMP-PDE delta, Protein p17, Retinal Rod Rhodopsin Sensitive cGMP 3' 5' Cyclic Phosphodiesterase Subunit delta)