Phosphodiesterase 7A, Recombinant, Human, GST-Tag (PDE7A)

Dry Ice

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Supplied as a liquid in 40mM Tris-HCl ( pH 8.0), containing 100mM NaCl, 2.2mM KCl, 4mM glutathione, 0.04% Tween-20, 20% glycerol and 3mM DTT.

Human recombinant PDE7A isoform a (GenBank Accession No. NM_002603), (a.a. 121-end) with N-terminal GST tag, expressed in a Baculovirus-infected Sf9 cell expression system.

Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Specific Activity:
≥ 180pmol/min/ug (lot-specific)

Unit Definition of Activity:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.

Assay for Activity:
Activity is assayed using Cat. # 137347 (PDE7A Assay Kit, BioAssay™).
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200uM cAMP, 2.5kU 5'-nucleotidase, and serial dilutions of PDE7A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Storage and Stability:
Store at -80°C. Product is stable for 6 months -80°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.