Phosphodiesterase 11A4, Recombinant, Human, GST-Tag (PDE11A4)

Dry Ice

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Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 0.04% Tween-20, 20% glycerol, 1.6mM glutathione and 3mM DTT.

Full length recombinant corresponding to human PDE11A4 with an N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. Genebank: BC112393.

Specific Activity:
As reported

Unit Definition:
1 unit is the amount of P4072-11D that will convert 1 pmole 3’, 5’-cAMP to 5’-AMP per minute at 37°C.

Assay Conditions:
10mM Tris-HCl, pH 7.4, 10mM MgCl2; 0.1mg/ml BSA, 0.05% Tween-20, 200uM cAMP and serial dilutions of PDE11A4 at 37ºC for 20 min. Quantified by 5 nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.

Applications:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Avoid repeated freezing and thawing. Store at -70°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.