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Human CD51 ELISA Kit PicoKine® Europäischer Partner

ArtNr BOS-EK2223
Hersteller Boster
Menge 96 wells/kit, with removable strips.
Kategorie
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity <47 pg/ml
Lieferbar
Manufacturer - Category
PicoKine® ELISA Kits
Storage Conditions
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name
ITGAV
Manufacturer - Specificity
Natural and recombinant human ITGAV
Background
Integrin alpha-V is a protein that in humans is encoded by the ITGAV gene. The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Immunogen
Expression system for standard: CHO; Immunogen sequence: F31-V992
Description
Human CD51 PicoKine® ELISA Kit (96 Tests). Quantitate Human CD51 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 47 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type
cell culture supernatants, serum and plasma (heparin, EDTA)
Sample Data
Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1, 500 | 3, 000 | 6, 000
O.D. | 0.031 | 0.069 | 0.143 | 0.315 | 0.692 | 0.926 | 1.425 | 2.054
Intra Assay Consistency & Inter Assay Consistency
Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean(pg/ml) | 179 | 236 | 2233 | 108 | 249 | 2866
Standard deviation | 6.01 | 14.18 | 86.64 | 4.36 | 19 | 95.15
CV(%) | 3.4% | 6% | 3.9% | 4% | 7.6% | 3.3%
Reproducibility
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 179 | 151 | 95 | 120 | 136 | 3.15 | 2.3%
Sample 2 | 236 | 204 | 241 | 308 | 247 | 12.81 | 5.2%
Sample 3 | 2233 | 1878 | 2332 | 2092 | 2134 | 93.24 | 4.4%
*number of samples for each test n=16.
Capture antibody and Detection antibody
a polyclonal antibody from goat|a biotinylated polyclonal antibody from goat
Kit Components
Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4
Standard
10 ng/tube
Anticoagulant
heparin or EDTA
Material Required But Not Provided
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Manufacturer - Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96 wells/kit, with removable strips.
Lieferbar: In stock
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