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Mouse MARCO ELISA Kit PicoKine®

Mouse MARCO ELISA Kit PicoKine®

ArtNr	BOS-EK2263
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Neuroscience Targets for Rodent Neurosciences ELISA Immunostaining
Applikationen	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	<150 pg/ml
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Gene Name	Marco
Manufacturer - Specificity	Natural and recombinant mouse Marco
Background	Macrophage receptor with collagenous structure (MARCO) is a protein that in humans is encoded by the MARCO gene. The protein encoded by this gene is a member of the class A scavenger receptor family and is part of the innate antimicrobial immune system. The protein may bind both Gram-negative and Gram-positive bacteria via an extracellular, C-terminal, scavenger receptor cysteine-rich (SRCR) domain. In addition to short cytoplasmic and transmembrane domains, there is an extracellular spacer domain and a long, extracellular collagenous domain. The protein may form a trimeric molecule by the association of the collagenous domains of three identical polypeptide chains.
Immunogen	Expression system for standard: NS0; Immunogen sequence: Q70-S518
Description	Mouse MARCO ELISA Kit PicoKine® (96 Tests). Quantitate Mouse MARCO in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 150 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum and plasma (heparin, EDTA).
Sample Data	Concentration (pg/ml) \| 0 \| 312 \| 625 \| 1, 250 \| 2, 500 \| 5, 000 \| 10, 000 \| 20, 000 O.D. \| 0.016 \| 0.045 \| 0.089 \| 0.178 \| 0.323 \| 0.767 \| 1.251 \| 1.873
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 555 \| 709 \| 7761 \| 438 \| 1039 \| 5548 Standard deviation \| 30.53 \| 44.88 \| 305.01 \| 27.81 \| 41.04 \| 220.81 CV(%) \| 5.5% \| 6.3% \| 3.9% \| 6.4% \| 4% \| 4%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 555 \| 600 \| 343 \| 464 \| 491 \| 21.29 \| 4.3% Sample 2 \| 709 \| 1133 \| 780 \| 1104 \| 932 \| 47.79 \| 5.1% Sample 3 \| 7761 \| 5570 \| 7930 \| 9513 \| 7694 \| 168.49 \| 2.2% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	20 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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BOS-EK2263-datasheet.pdf

BOS-EK2263-safety-datasheet.pdf