Human PDGFRA ELISA Kit PicoKine®

PicoKine® ELISA Kits

PDGFRA

Platelet-derived growth factor receptor A, also termed CD140a, is a receptor located on the surface of a wide range of cell types. The protein is encoded in the human by the PDGFRA gene. This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Human PDGFRA ELISA Kit PicoKine® (96 Tests). Quantitate Human PDGFRA in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 45 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1, 500 | 3, 000 | 6, 000
O.D. | 0.029 | 0.099 | 0.186 | 0.397 | 0.863 | 1.273 | 1.579 | 2.097

Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%)
Sample 1 | 127 | 183 | 95 | 142 | 137 | 4.39 | 3.2%
Sample 2 | 317 | 299 | 328 | 202 | 287 | 9.43 | 3.3%
Sample 3 | 2663 | 1607 | 1919 | 1566 | 1939 | 132.42 | 6.8%
*number of samples for each test n=16.

Description|Quantity
Pre-coated 96-well strip microplate|1
Standard|2 vials
Biotinylated antibody (100x)|100ul
Avidin-Biotin-Peroxidase Complex (100x)|100ul
Sample Diluent|30ml
Antibody Diluent|12ml
Avidin-Biotin-Peroxidase Diluent|12ml
Color Developing Reagent (TMB)|10ml
Stop Solution|10ml
Wash Buffer (25x)|20ml
Adhesive plate sealers|4

heparin or EDTA

There is no detectable cross-reactivity with other relevant proteins.