Vergleich

GENLISA Human Lens Epithelium Derived Growth Factor (LEDGF) ELISA

ArtNr KRIS-KBH4576
Hersteller Krishgen Biosystems
Menge 1 x 96 wells, break-apart wells
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Human (Homo sapiens)
Sensitivity 0.05 ng/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias PSIP1,p75,p52,DFS70,PAIP,PSIP2,PC4 And SFRS1 Interacting Protein 1,CLL-associated antigen KW-7,Dense fine speckles 70 kDa protein,Transcriptional coactivator p75/p52,Lens Epithelium Derived Growth Factor
Lieferbar
Manufacturer - Type
Sandwich ELISA, Double Antibody
Manufacturer - Category
Human ELISA
Shipping Temperature
Shipped at Room Temperature or in gel pack to maintain temperature at 2-8 degrees Celsius
Storage Conditions
The kit (2-8 Degree Celsius) and its components are to be stored as indicated in the IFU (instructions for use). To ensure quality of your results, if all the wells in the kit are not being used, store the unused wells in the foil pouch containing the desiccant pack, well sealed and store at the indicated temperature. Similary aliquot and store the reconstituted standard at -20 Degree Celsius for long term storage (maximum 6 months from reconstitution) and to avoid deterioration in the activity.
HSN Code
38220090
Short Description
Enzyme Immunoassay for the estimation of Lens Epithelium Derived Growth Factor (LEDGF) ELISA in serum, plasma, cell culture supernatant and other biological samples.
Manufacturer - Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. For tear samples, please connect with us to discuss further.
Description
The GENLISA Human Lens Epithelium Derived Growth Factor (LEDGF) ELISA includes features like:
Ready to use protocol with break-apart wells for ease of use
Standardisation and High Reproducibility
Lot to Lot Consistency
Accuracy and Precision

Validated against seven points for a “GOLD RING” Standard Quality ELISA the benchmark sign for Krishgen Quality. The GENLISA ELISAs are used for assessing the specific biomarker in samples analytes which may be human serum, plasma, biological fluids and cell culture supernatant and uses monoclonal antibodies to ensure a high degree of specificity and reagents of superior quality to offer a sensitive assay. For project based larger number ofs, talk to us to offer you our validation guide for the same
Standards Available
0; 0.156; 0.3125; 0.625; 1.25; 2.5; 5; 10 ng/ml
Calibration Curve
8 point; standards available
Interference
Preparations of the known factors were assayed for interference. No significant interference was observed.
Old Assay Principle
The assay uses monoclonal antibody against the analyte and is highly specific as a capture protein.
Principle of Assay
This ELISA is a sandwich immunoassay. Antibodies are coated on 96 well plates. The antigen protein present in sample and standard respectively bind to the coated wells. The wells are washed and an antibody:HRP Conjugate is added which binds to the bound complex in the well. Washing is performed to remove any unbound material. TMB substrate is added and the enzyme reaction is stopped by dispensing of stop solution into the wells. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of antigen protein present in the standard or samples.
Detection Method
Colorimetric, Absorbance measured at 450nm
Conjugate-Enzyme Reaction
Uses stable HRP Enzyme Conjugate with (single component) TMB Chromogenic Substrate for color development.
Regulatory Status
Research Use Only.
ELISA Interpretation
Quantitative Results Intepretation, 4PL (2nd order) or Cubic Spline Graph. Software (MS Excel) available, on request, to support interpretation of results.
Shelf Life Available
8 -10 months at time of shipping
Manufacturer - Research Area
Immune molecule; Cell Biology; Immunology
Disclaimer
The data indicated herein with specifications are changed from time to time at time of production of the assay. We request you to confirm the specifications including the assay range and procedure as per the most current IFU (instructions for use) accompanying the assay kit.

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 x 96 wells, break-apart wells
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen