resDetect™ Universal Protein A Quick ELISA kit

The kit is developed for the detection of natural or structurally conserved recombinant forms of Protein A and alkaline-resistant Protein A variants, such as MabSelect SuRe™ , MaXtar® ARPA ligand (Bio-Link Co.), etc. in bioprocess manufacturing applications. It is used as a universal protein A and variants ligand detection tool to aid in optimal antibody purification process development and in routine quality control of in-process streams as well as final product.

Blue ice

The Protein A ELISA kit is used to measure the levels of protein A by employing a standard sandwich-ELISA format. The micro-plate in the kit has been pre-coated with a chicken anti-protein A polyclonal antibody.
Firstly, the standard samples provided in kit and your samples are heated in a dry heating block or boiling water bath to denature and precipitate the product antibodies, after a centrifugation step to pellet the denatured product antibody, add the standard samples and your samples supernatant to the plate, then add the Biotin-Anti-Protein A Antibody to the plate and form Antibody-antigen (Protein A) - biotinylated antibody complex, incubate and wash the wells.
Next add Horseradish peroxidase conjugated streptavidin (Streptavidin-HRP) to the plate, incubate and wash the wells to remove any unbound reactants.
At last, load the tetramethylbenzidine (TMB) substrate into the wells and monitor a blue color. The reaction is stopped by the addition of a stop solution and the color turns yellow. The intensity of the absorbance can be measured at 450nm and 630nm on a microtiter plate reader. The OD Value reflects the amount of protein A.

resDetect™ Universal Protein A Quick ELISA kit

protein A (SuRe)