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BAP1 Knockout 293T Cell Line, Homozygous

BAP1 Knockout 293T Cell Line, Homozygous

Hersteller	Abclonal
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Specific against	other
Menge	1 Strips
ArtNr	RM01958-1Strips
eClass 6.1	32160890
eClass 9.0	32160890
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AntigenSeq	Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO₂ condition. 1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible. 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37℃ incubator with 5% CO₂. 7. A subcultivation ratio of 1:2-1:4 is recommended.
BackGround	This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013]
GeneID(Human)	8314
GeneSymbol	BAP1
Imminogen	Dry ice
ProteinWeight	BAP1
RecommandDilutionA	1 vial parental cell line and 1 vial knockout cell line
Route	Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Storage	Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
protein size	BAP1

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Menge: 1 Strips

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