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IL1B Knockout A549 Cell Line, Homozygous

IL1B Knockout A549 Cell Line, Homozygous

Hersteller	Abclonal
Kategorie	Cell Lines & Cell Culture Cell Lines
Typ	Cell line
Specific against	other
Menge	1 strips
ArtNr	RM02641-1strips
eClass 6.1	32160890
eClass 9.0	32160890
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AntigenSeq	Upon arrival, it should be maintained in DMEM medium with 10%(v/v) fetal bovine serum and 100U penicillin-streptomycin, at 37℃ with 5% CO₂ condition. 1. Thaw the vial in 37℃ water bath , and shake it to melt as soon as possible. 2. Transfer the cell suspension to a 15mL conical tube with pre-warmed 5mL complete me- dium and centrifuge 1000rpm for approximately 5 minutes at room temperature. 3. Remove and discard the supernatant. 4. Resuspend the cell pellet with 1mL pre-warmed complete medium and seed in 10cm dish. 5. Add 8-10mL of complete medium. 6. Incubate the culture at 37℃ incubator with 5% CO₂. 7. A subcultivation ratio of 1:2-1:4 is recommended.
BackGround	The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
GeneID(Human)	3553
Imminogen	Dry ice
ProteinWeight	IL1B
RecommandDilutionA	1 vial parental cell line and 1 vial knockout cell line
Route	Complete medium supplemented with 40%FBS and 10% (v/v) DMSO.
Storage	Stored in liquid nitrogen for a long time less than -130℃. Minimizing freeze-thaw cycles.
protein size	IL1B

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Menge: 1 strips

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