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Active Recombinant Mouse FGF-2/bFGF Protein

Hersteller Abclonal
Kategorie
Typ Proteins Recombinant
Specific against Mouse
Menge 10 ug
ArtNr RP01345-10ug
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Description
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.
Background
Basic fibroblast growth factor (bFGF), also known as FGF2, is a member of the fibroblast growth factor (FGF) family. It is a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in remodeling damaged tissue, such as ulcer healing, vascular repair, traumatic brain injury (TBI). bFGF is a critical component of human embryonic stem cell culture medium. In addition, bFGF protein is a heparin-binding cationic protein involved in a variety of pathological conditions including angiogenesis and solid tumour growth. Thus, bFGF is regarded as a target for cancers chemopreventive and therapeutic strategies.
Route
C-His
Manufacturers Category
Proteins
Endotoxin
<0.1EU/μg
Immunogen
Ala11-Ser154
Storage
Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Manufacturers Research Area
Growth Factor
Gene Symbol
FGF-2/bFGF
Protein Formulation
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Protein Description
Recombinant Mouse FGF-2/bFGF Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala11-Ser154) of mouse FGF2/FGFB (Accession #NP_032032.1) fused with 6×His tag at the C-terminus.
Protein Bio Activity
Active Recombinant Mouse FGF2 was added during DP cell culture. The immunofluorescence identification showed that 1 ng/mL FGF2 could maintain the cellular characteristics of DP cells. The red part is the fluorescently labeled PDFGRA antibody, and the blue-cyan part is the DAPI-stained cells.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: In stock
lieferbar

Lieferung vsl. bis 28.05.2024 

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