Hersteller |
Abclonal
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
Human |
Menge |
20 ug |
ArtNr |
RP01358-20ug |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Description |
Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Background |
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. |
Route |
C-hFc&His |
Manufacturers Category |
Proteins |
Endotoxin |
<0.1EU/μg |
Immunogen |
Thr21-Ile192 |
Storage |
Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. |
Manufacturers Research Area |
Immune Checkpoint, Bio-Markers & CD Antigens, TNF family, Biosimilar Drug Targets |
Gene Symbol |
TNFRSF7/CD27 |
Protein Formulation |
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. |
Protein Description |
Recombinant Human TNFRSF7/CD27 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr21-Ile192) of human CD27/TNFRSF7 (Accession #NP_001233.1) fused with a Fc, 6×His tag at the C-terminus. |
Protein Bio Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human CD27 at 2 μg/mL (100 μL/well) can bind Human CD70 with a linear range of 0.781-0.885 ng/mL. |
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