Recombinant Human DNA Topoisomerase-I 70kDa

TOP1 is an important nuclear enzyme that interconverts supercoiled DNA to the necessary topological conformations for standard DNA replication and transcription. TOP1 is the target antigen for TOP1 autoantibodies. TOP1 antibodies are a specific marker in scleroderma patients (specificity 98-100%) and are related with the existence of diffuse skin involvement and pulmonary fibrosis. In human tissues top1 enzyme is primarily synthesized as a protein with a molecular weight of 100-kDa. Most of this precursor is then proteolytically processed to a 70-kDa size, from which the TOP1 antigen has derived its name.

Sf9 insect cells.

Greater than 80% as determined by SDS-PAGE.

Store at 4C if entire vial will be used within 2-4 weeks. Store, frozen at -20C for longer periods of time.
Avoid multiple freeze-thaw cycles.