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Recombinant Human Guanylate Kinase 1, Active Europäischer Partner

ArtNr pka-309-2ug
Hersteller ProSpec
Menge 2ug
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products ELK-1
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Synonyms
ETS domain-containing protein Elk-1, ELK1, Elk-1
Introduction
Elk-1 is a transcription factor that is activated by mitogen-activated protein kinases (MAPKs) and whose main function is the regulation of growth-related proteins in response to extracellular stimuli. Because of the importance of Elk-1 to the regulation of successful cell growth, accurate monitoring of Elk-1 in cells, tissues and animals is crucial for many biomedical research and drug development projects.
Description
ELK-1 (amino acids 307-428) Human Recombinant is produced in E.Coli as a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and a c-terminal myc tag (EQKLISEEDL), and having a molecular mass of 60 kDa.
Elk-1 is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
ELK-1 is supplied as lyophilized freeze dry powder without additives.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Biological Activity
Recombinant human Elk-1 is phosphorylatable in vitro, using either recombinant active ERK1/2, or with immunoprecipitated ERK1/2 from stimulated cells. This phosphorylation can be monitored by Western blot analysis. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.
Storage
Store at 2-8C if entire vial will be used within 1-2 weeks. Store, frozen at -20C for longer periods of time. Avoid multiple freeze-thaw cycles.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2ug
Lieferbar: In stock
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