Human CD20 protein, His tag (Animal-Free)

The recombinant human CD20 protein consists of 91 amino acids and predicts a molecular mass of 10.6 KD. It migrates as an approximately 23 KD band in SDS-PAGE under reducing conditions due to glycosylation.

>90% as determined by SDS-PAGE.

Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CD20 protein alpha protein using the buffer we provided not less than 100 ug/mL, which can then be further diluted to other aqueous solutions.

The product is shipped at ambient temperature.

B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. CD20/MS4A1may be involved in the regulation of B-cell activation and proliferation. Defects in CD20/MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5). CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

CD80; Activation B7-1 antigen; B7; BB1; CD28LG1; CD28LGB7-1 antigen; T-lymphocyte activation antigen CD80

CD20