Heat-Activated DNA Polymerase with Red Dye Mix

Heat-Activated DNA Polymerase with Red Dye Mix

Abbexa's Heat-Activated DNA Polymerase with Red Dye Mix is a complete ready-to-use 2X reaction mix containing a heat-activated thermostable enzyme with improved specificity as compared to standard polymerases, by eliminating all non-specific priming and the formation of primer-dimers. It is inactive at room temperature, allowing for reaction set-up at room temperature, and therefore requires activation by heat treatment for 10 minutes prior to PCR cycling. It also contains an additional inert red dye that permits easy visualization and direct loading onto a gel. No loading buffer is required as the mix is of sufficiently high density to sink to the bottom of the gel. The mix has been developed to perform PCR assays of many common genomic and cDNA templates; simply add water, template and primers. It dramatically reduces the time required to set up reactions, thereby minimizing the risk of contamination. Greater reproducibility is ensured, by reducing the number of pipetting steps that can lead to errors. The 500 rxns size is provided as 10 × 1.25 ml Heat-Activated DNA Polymerase with Red Dye Mix, and 1.2 ml 50 mM MgCl₂ solution for optional optimization of reaction conditions.

The exact formulation is proprietary.