Recombinant SARS-CoV-2 (2019-nCoV) NSP16 / 2'-O-Methyltransferase (His Tag)

Stable for up to 1 year from date of receipt at -20°C to -80°C. It is recommended to store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

2'-O-Methyltransferase/nsp16 is one of 15 chains cleaved from Replicase polyprotein 1ab. 2'-O-Methyltransferase mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.Nonstructural protein 16 (NSP16) / viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting on the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral drugs development which can against SARS-CoV-2, SARS-CoV, MERS-CoV or other RNA and DNA viruses.

Replicase polyprotein 1ab