Hersteller |
ProSci
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Format |
Lyophilized |
Applikationen |
WB |
Menge |
0.1 mg |
ArtNr |
PRS-96-560-0.1mg |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Applications |
This recombinant protein can be used for WB. For research use only. |
Applications |
This recombinant protein can be used for WB. For research use only. |
Buffer |
50 mM tris, 100 mM glycine, pH7.5 |
Storage Conditions |
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. |
Predicted Molecular Weight |
49.8 kDa |
Purity |
>95% as determined by SDS-PAGE. |
Background |
Noggin is also known as NOG,SYM1, SYNS1 and is a secreted homodimeric glycoprotein whose scaffold contains a cystine-knot topology similar to that of BMPs.Secreted Noggin probably remains close to the cell surface due to its binding of heparincontaining proteoglycans.Noggin inhibits TGF-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion. During embryogenesis, Noggin antagonizes specific BMPs at defined times, for example, during neural tube, somite and cardiomyocyte growth and patterning. During skeletal development, Noggin prevents chondrocyte hyperplasia, thus allowing proper formation of joints. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons Noggin also appears to maintain adult stem cell populations in vivo, for example, maintaining neural stem cells within the hippocampus. |
1St Image Caption3 |
The purity of rh Noggin Fc Chimera was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue. |
Background References 1 |
Valenzuela, D.M. et al. 1995, J. Neurosci. 15:6077. |
Background References 2 |
Groppe, J. et al. 2002, Nature 420:636. |
Background References 3 |
Itsykson, P. et al. 2005, Mol. Cell. Neurosci. 30:24. |
Background References 4 |
Xu, R.H.et al. 2005, Nat. Methods 2:185. |
Accession # |
NP_005441 |
Ncbi Gene Id # |
9241 |
Ncbi Official Symbol |
NOG |
Ncbi Official Full Name |
noggin |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q13253 |
Fusion Tag |
C-Fc Tag |
Sequence |
Gln 28 - Cys 232 |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.