Hersteller |
ProSci
|
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Format |
Lyophilized |
Applikationen |
WB, ELISA |
Menge |
0.2 mg |
ArtNr |
PRS-96-987-0.2mg |
eClass 6.1 |
34160400 |
eClass 9.0 |
42020190 |
Lieferbar |
|
Applications |
This recombinant protein can be used for E, WB. For research use only. |
Applications |
This recombinant protein can be used for E, WB. For research use only. |
Buffer |
PBS, pH7.4 |
Storage Conditions |
Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. |
Predicted Molecular Weight |
15.9 kDa |
Purity |
>95% as determined by SDS-PAGE.
Endotoxin level is less than 1.0 EU per ug by the LAL method. |
Background |
CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis. |
1St Image Caption3 |
Mouse CTLA-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. |
Background References 1 |
Waterhouse P, et al., 1995, Science 270 (5238): 985–8. |
Background References 2 |
Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602. |
Background References 3 |
Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26. |
Accession # |
NP_033973 |
Ncbi Gene Id # |
12477 |
Ncbi Official Symbol |
CTLA-4 |
Ncbi Official Full Name |
cytotoxic T-lymphocyte protein 4 isoform 1 precursor |
Ncbi Organism |
Mus musculus |
Swissprot # |
P09793 |
Fusion Tag |
His Tag |
Sequence |
Glu 36 - Phe 162 |
Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Mouse B7-1, Fc Tag at 2 ug/mL (100 ul/well), can bind Mouse CTLA-4, His Tag with a linear range of 0.02-0.8 ng/mL. |
Peptide Sequence |
Glu 36 - Phe 162 |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.