TransScript® II Reverse Transcriptase[M-MLV,RNaseH-]（High Temperature RT）

RT-PCR

TransScript® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability. The enzyme is active at up to 55℃. It provides higher specificity, higher yield and more full-length cDNA products.

• Increased thermostability for more full-length cDNA products.
• Reaction temperature at 42℃-55℃.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.


Applications
• First-strand cDNA synthesis, cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template


Storage
at -20 ℃ for two years


Shipping
Dry ice (-70 ℃)