TRIM21, Recombinant, Human, aa1-285, His-Tag (52KD Ribonucleoprotein Autoantigen Ro, Ro-52, Trim21, RNF81, RO52, SSA, SSA1)

Blue Ice

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Supplied as a liquid in 50mM Tris buffer, pH 8.5, 100mM sodium chloride, 1mM EDTA, 2mM DTT, 0.02% SDS.

TRIM21 forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination.

Recombinant protein corresponding to aa1-285 from human TRIM21, fused to His-tag expressed in E. coli. Swiss/Uniprot: P19474

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.