PADI-3, Recombinant, Human, aa1-664 (Peptidyl arginine deiminase, type III)

Dry Ice

75

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 80ug/ml FLAG, 20% glycerol.

Human PADI-3, also known as Peptidyl arginine deiminase, type III, (GenBank Accession No. NM_016233), 1-664(end), with C-terminal FLAG-tag, MW= 75kD, expressed in a Baculovirus infected Sf9 cell expression system.

Source:
Recombinant protein corresponding to aa1-664 from human PADI-3 at C-terminal, fused to FLAG-tag expressed in Baculovirus infected Sf9.

Molecular Weight:
~75kD

Specific Activity:
22pmole/min/ug

Assay Conditions:
Enzyme reaction took place in a buffer (50mM Tris, pH 7.4, 50mM NaCl, 10mM CaCl2, and 2mM DTT) containing PADI and 10mM BAEE as a substrate for 25 min at 37°C.

Application:
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap