NAD Synthetase, Recombinant, Bacillus subtilis (NH(3)-dependent NAD(+) Synthetase, General Stress Protein 38, GSP38, Spore Outgrowth Factor B, EC6.3.1.5)

Dry Ice

30, 4

Supplied as a liquid in 50mM TRIS-HCl, pH 8.0, 75mM sodium chloride, 5mM DTT, 5% glycerol.

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore. Catalytic activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.

Source:
Recombinant protein corresponding to full-length Bacillus subtilis NAD Synthetase, expressed in E. coli.

Molecular Weight:
~30.4kD

Biological Activity:
~0.3U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol NAD per min.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.