NAPRT1, Recombinant, Human, His-Tag (Nicotinate Phosphoribosyltransferase, NAPRTase, FHA-HIT-interacting Protein, Nicotinate Phosphoribosyltransferase Domain-containing Protein 1, FHIP, EC 2.4.2.11)

Dry Ice

58

Supplied as a liquid in 50mM HEPES/KOH, pH 7.5, 300mM potassium chloride, 10mM DTT.

Human nicotinate phosphoribosyltransferase (NAPRTase) is localized in the cytoplasm and is involved in biological processes such as NAD biosynthetic and metabolic processes, nicotinamide metabolic process, nicotinate nucleotide salvage, response to oxidative stress and H2O-soluble vitamin metabolic process. It functions by catalyzing the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN) and is essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. It is a crucial factor in the NAD+ biosynthesis pathway.

Catalytic activity:
B-nicotinate D-ribonucleotide + diphosphate = nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate.

Source:
Recombinant protein corresponding to human NAPRTase, fused to His-tag at N-terminal, expressed in E. coli.

Molecular Weight:
~58kD

Biological Activity:
~0.27U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol nicotinic acid mononucleotide (NaMN) per min.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.