Tim-3 (Human):Fc (Mouse), Recombinant, aa22-200 (TIM3, TIMD3, Hepatitis A Virus Cellular Receptor 2, HAVcr-2, T Cell Immunoglobulin and Mucin Domain-containing Protein 3)

Molecular Biology / MB-Infectious Disease

-20°C

Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O. Add 1X PBS to the desired protein concentration.

The TIM (T cell/transmembrane, immunoglobulin and mucin) family plays a critical role in regulating immune responses, including allergy, asthma, transplant tolerance, autoimmunity and the response to viral infections. The unique structure of TIM immunoglobulin variable region domains allows highly specific recognition of phosphatidylserine (PtdSer), exposed on the surface of apoptotic cells. Tim-3, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. TIM-3 is preferentially expressed on Th1 and Tc1 cells, and generates an inhibitory signal resulting in apoptosis of Th1 and Tc1 cells. TIM-3 is also expressed on some dendritic cells and can mediate phagocytosis of apoptotic cells and cross-presentation of antigen. Tim-3 functions to inhibit aggressive Th1-mediated auto- and alloimmune responses. Tim-3 pathway blockade by administration of Tim-3:Fc fusion protein accelerates diabetes in nonobese diabetic mice, causes hyperproliferation of Th1 cells and Th1 cytokine release in an experimental autoimmune encephalomyelitis (EAE) model and prevents acquisition of transplantation tolerance induced by costimulation blockade.

The extracellular domain of human Tim-3 (aa22-200) is fused to the N-terminus of the Fc region of mouse IgG2a. expressed in CHO cells.

Biological Activity:
Measured by its binding ability in a functional ELISA.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.