TIP60, Recombinant, Human, His-Tag (Histone Acetyltransferase HTATIP, Histone Acetyltransferase KAT5, Lysine Acetyltransferase 5, 60kD Tat-interactive Protein, Tip60, HIV-1 Tat Interactive Protein, cPLA(2)-interacting Protein)

Molecular Biology / MB-Enzymes, Acetyltransferase

-70°C

Supplied as a liquid in 50mM TRIS-HCl, pH 7.5, 100mM sodium chloride, 0.2% NP-40, 50mM imidazole, 10% glycerol.

TIP60 is the catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex is required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. TIP60 directly acetylates and activates ataxia-telangiectasia mutated (ATM). In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.

Recombinant protein corresponding to human TIP60, fused to His-tag, expressed in Sf21 cells.

Biological Activity:
~100-200ng are required for standard HAT assay.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TIP60, Recombinant, Human, His-Tag (Histone Acetyltransferase HTATIP, Histone Acetyltransferase KAT5, Lysine Acetyltransferase 5, 60kD Tat-interactive Protein, Tip60, HIV-1 Tat Interactive Protein, cPLA(2)-interacting Protein)