Zmp1, Recombinant, M. tuberculosis, His-Tag (Zinc Metalloprotease 1, Endopeptidase, Rv0198c)

Dry Ice

73, 9

Supplied as a liquid in 10mM TRIS-HCl, pH 8.0.

M13 endopeptidases are single-pass, transmembrane zinc-metallopeptidases responsible in human for the regulation of the biological activity of many hormones and peptides and are involved in many important processes such as blood pressure regulation (Neprilysin or NEP1), cardiovascular development (Endothelin Converting Enzyme-1 or ECE-12), prevention of hemolytic reaction (KELL) and phosphate homeostasis (PHEX). Zmp1 is a soluble enzyme member of the M13 endopeptidases family that plays a key role in Mycobacterium tuberculosis pathogenicity with a still unknown mechanism. Recently, it was shown to suppress inflammasome activation by inhibiting caspase-1 activation, thus preventing processing of pro-IL-1beta into IL-1beta and the consequent phagosome maturation. Prefered cleavage sequence is PxxF.

Source:
Recombinant protein corresponding to full-length Mycobacterium tuberculosis Zmp1, fused to His-tag at N-terminal, expressed in E. coli.

Molecular Weight:
~73.9kD

Biological Activity:
~0.5U/mg protein. Active towards the generic fluorogenic substrate for matrix metalloproteinases MMP2/MMP7.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.