EDIM/Rotavirus Capsid Protein 6, Recombinant, Mouse (VP6, EDIM-VP6), Western Blot Positive Control

Blue Ice

46, 5

Supplied as a lyophilized powder in PBS, 0.05% azide. Reconstitute with 100ul sterile PBS.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. Animals or animal-derived products are transported from one part of the world to another in a matter of days. So there is great potential for the diseases to spread very quickly. Many infections are asymptomatic and without any overt clinical symptoms. Detection of microbial infections has relied largely on serological screening and presence of microbial antigens or antibodies. Diarrhea in young laboratory mice is often caused by mouse rotavirus, also called epizootic diarrhea of infant mice (EDIM). This virus is highly contagious and is transmitted via contaminated bedding, airborne dust, and through contact with infected mice. There is no evidence of transplacental infection. These animals present with watery, mustard-colored stools, lethargy, and distended abdomens. If the impacted fecal material is not removed spontaneously or deliberately, the animals will die. Rotavirus infections are the primary causes of several gastroenteritis in young children and are the cause of nearly one million deaths worldwide each year. Diagnosis is usually based on serology, via ELISA or IFA or both. EDIM or rotavirus is a genus of dsRNA virus in the family Reoviridae. There are five species of this virus (A-E). Rotavirus A, the most common, causes more than 90% of infections in humans. Rotaviruses infect the young of many species of animals and they are a major cause of diarrhoea in wild and reared animals worldwide. As a pathogen of livestock, notably in young calves and piglets, rotaviruses cause economic loss to farmers because of costs of treatment associated with high morbidity and mortality rates. The genome of rotavirus consists of 11 unique double helix molecules of RNA which are 18.5kb in total. Each helix, or segment, is a gene, numbered 1 to 11 by decreasing size. Each gene codes for one protein, except genes 9, which codes for two. The RNA is surrounded by a three-layered icosahedral protein capsid. There are six viral structural capsid proteins (VP1-4, VP6-7) that form the virus particle (virion). In addition to the VPs, there are six nonstructural proteins (NSPs), that are only produced in cells infected by rotavirus (NSP1- 6). VP6 forms the bulk of the capsid. It is highly antigenic and can be used to identify rotavirus infections. VP6 protein of the mouse rotavirus strain EDIM are able to elicit protection against rotavirus shedding in the adult mouse model. VP6-based human vaccines are in active clinical trials.

Source:
Recombinant protein corresponding to 397aa from full-length mouse EDIM-VP6, fused to His-tag, expressed in E. coli.

Molecular Weight:
~46.5kD

Applications:
Suitable for use in ELISA, Positive Control for Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:10-50K; using 50-100ng antigen/well
Western Blot: 1:500-2K
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.