IKKB-beta, Recombinant, Human, GST-His-IKK-B, Active

Dry Ice

120

Supplied as a liquid in 50mM Tris-HCl, pH 8.0, 100mM sodium chloride, 5mM DTT, 4mM reduced glutathione, 20% glycerol.

Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting..

IKK-beta also known as inhibitor of nuclear factor kappa-B kinase subunit beta is a protein that in humans is encoded by the IKBKB (inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta) gene. IKK-beta is an enzyme that serves as a protein subunit of IB kinase, which is a component of the cytokine- activated intracellular signaling pathway involved in triggering immune responses. Its activity causes activation of a transcription factor known as Nuclear Transcription factor kappa-B or NF-B. Activated IKK-beta phosphorylates a protein called the inhibitor of NF-beta B, IB (IkB), which binds NF-B to inhibit its function. Phosphorylated IB is degraded via the ubiquitination pathway, freeing NF-B, and allowing its entry into the nucleus of the cell where it activates various genes involved in inflammation and other immune responses.

Source:
Recombinant protein corresponding to human IKKB-beta, N-terminally fused to GST-His6-Thrombin cleavage site, expressed in sf9 cells.

Molecular Weight:
~120kD

Activity:
~6pmol/ug min

Applications:
Suitable for use in Western Blot and Kinase Assays. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.