Kilham Virus Capsid Protein VP2, Recombinant, Rat, His-Tag (KRV)

Blue Ice

65

Supplied as a liquid in 50mM Tris, pH 8, 0.25M sodium chloride, 5mM beta-mercaptoethanol, 0.5mM ETDA, 0.25M imidzazole, 8M urea.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. Animals or animal-derived products are transported from one part of the world to another in a matter of days. So there is great potential for the diseases to spread very quickly. Many infections are asymptomatic and without any overt clinical symptoms. Detection of microbial infections has relied largely on serological screening and presence of microbial antigens or antibodies. Parvovirus casually applied to all the viruses in the Parvoviridae taxonomic family and also the taxonomic name of the Parvovirus genus within the Parvoviridae family. Parvoviruses (from Latin parvus meaning small) are typically linear, non- segmented single-stranded DNA viruses, with an average genome size of 5Kb. Parvoviruses tend to be specific about the taxon of animal they will infect. The viral capsid of a parvovirus is made up of two or three proteins, known as VP1-3 that form an icosahedral structure that is resistant to acids, bases, solvents and temperature up to 50°C. Structural protein (NS1-2) are conserved and involved in transcription and virus replication. Capsid proteins (VP1-3) exhibit heterogeneity among different parvoviruses. Parvovirus diagnosis is by serology and ELISA. MPV is most pathogenic for haematopoietic cells than mouse parvoviruses (MPVs): Species: Minute virus of mouse (MVM) or mice minute virus (MMV), Kilham rat virus (KRV), Rat H-1 virus (Toolan’s virus), mouse parvovirus (MPV), hamster (HaPV) and rat parvovirus (RPV-1a). Natural hosts: Vertebrates. Transmission: Respiratory, oral droplets of fecal oral-route. Geography: Worldwide. The Kilham rat virus (KRV) is a parvovirus originally isolated from a rat sarcoma in the late 1950s. The clinical signs associated with a natural KRV infection include fetal resorption in dams, runting, ataxia, cerebellar hypoplasia and jaundice in suckling rats, and sudden death, scrotal cyanosis, abdominal swelling and dehydration in juvenile rats. The ability of this virus to produce persistent infections has resulted in a high frequency of contamination of cell cultures and transplantable-tumor system. In addition, the virus may interfere with research in other ways. The remarkable resistance to environmental conditions determines the importance of the detection and control of this agent, especially in the laboratory animal production. According to a study, the seroprevalence varied between 27.8% and 75%. KRV produces intranuclear inclusion bodies in epithelial connective tissue and fibroblastic rat embryo cells. KRV causes highly pathogenic infections, especially in liver and cerebellum. KRV is a single stranded DNA virus. It shares homology with MVM and MPV. KRV viral protein 2 (KRV-VP2, 582aa) is one of the major antigenic protein.

Source:
Recombinant protein corresponding to 582aa from full-length rat KRV-VP2, fused to His-tag, expressed in E. coli.

Molecular Weight:
~65kD

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng antigen/well
Western Blot: load 100-200ng/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.