Kilham Virus Capsid Protein VP2, Recombinant, Rat, His-Tag, Positive Control for Western Blot (KRV)

Blue Ice

65

Supplied in SDS-PAGE sample buffer (reduced). Add 5ul of fresh 2x sample buffer/10ul of the solution prior to heating and loading on gels.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. Animals or animal-derived products are transported from one part of the world to another in a matter of days. So there is great potential for the diseases to spread very quickly. Many infections are asymptomatic and without any overt clinical symptoms. Detection of microbial infections has relied largely on serological screening and presence of microbial antigens or antibodies.

The Kilham rat virus (KRV) is a parvovirus originally isolated from a rat sarcoma in the late 1950s. The clinical signs associated with a natural KRV infection include fetal resorption in dams, runting, ataxia, cerebellar hypoplasia and jaundice in suckling rats, and sudden death, scrotal cyanosis, abdominal swelling and dehydration in juvenile rats. The ability of this virus to produce persistent infections has resulted in a high frequency of contamination of cell cultures and transplantable-tumor system. In addition, the virus may interfere with research in other ways. The remarkable resistance to environmental conditions determines the importance of the detection and control of this agent, especially in the laboratory animal production. According to a study, the seroprevalence varied between 27.8% and 75%.

KRV produces intranuclear inclusion bodies in epithelial connective tissue and fibroblastic rat embryo cells. KRV causes highly pathogenic infections, especially in liver and cerebellum. KRV is a single stranded DNA virus. It shares homology with MVM and MPV. KRV viral protein 2 (KRV-VP2, 582aa) is one of the major antigenic protein.

Source:
Recombinant protein corresponding to 582aa from full-length rat KRV-VP2, fused to His-tag, expressed in E. coli.

Molecular Weight:
~65kD

Applications:
Suitable for use as Positive Control for Western Blot. It is not suitable for ELISA or other applications where native protein is required. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.