Minute Virus Capsid Protein VP2, Recombinant, Mouse, His-Tag (MVM-VP2)

Blue Ice

65

Supplied as a liquid in 50mM Tris, pH 8, 0.25M sodium chloride, 5mM beta-mercaptoethanol, 0.5mM ETDA, 0.25M imidzazole, 8M urea.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. So there is great potential for the diseases to spread very quickly.
Parvoviruses (from Latin parvus meaning small) are typically linear, non-segmented single-stranded DNA viruses, with an average genome size of 5Kb. The viral capsid of a parvovirus is made up of two or three proteins, known as VP1-3 that form an icosahedral structure that is resistant to acids, bases, solvents and temperature up to 50°C. Structural protein (NS1-2) are conserved and involved in transcription and virus replication. Capsid proteins (VP1-3) exhibit heterogeneity among different parvoviruses. Parvovirus diagnosis is by serology and ELISA. MPV is most pathogenic for haematopoietic cells than mouse parvoviruses (MPVs): Species: Minute virus of mouse (MVM) or mice minute virus (MMV), Kilham rat virus (KRV), Rat H-1 virus (Toolan’s virus), mouse parvovirus (MPV), hamster (HaPV) and rat parvovirus (RPV-1a). Natural hosts: Vertebrates.

Minute virus of mice (MVM) and mouse parvovirus (MPV or MPV- 1) are among the most prevalent infectious agents detected in contemporary laboratory mouse colonies, with ~45 % of USA research institutions harboring these infectious agents and MPV being among the most prevalent viruses detected in research mice. Various clinical disease syndromes in mice have been associated with MVM infection and both MVM and MPV can have deleterious effects on research due to in vitro and in vivo immunomodulatory effects and contamination of cell cultures and tissues originating from mice. As a result, mouse parvovirus infections comprise one of the most significant infectious disease problems encountered in contemporary laboratory animal research facilities.

Source:
Recombinant protein corresponding to 587aa from full-length mouse MVM-VP2, fused to His-tag, expressed in E. coli.

Molecular Weight:
~65kD

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng antigen/well
Western Blot: load 100-200ng/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.