Pneumonia Virus Nucleoprotein, Recombinant, Mouse, His-Tag (PVM-NP)

Blue Ice

44

Supplied as a liquid in 50mM Tris, pH 8, 0.25M sodium chloride, 5mM beta-mercaptoethanol, 0.5mM ETDA, 0.25M imidzazole, 8M urea.

Animals, just like humans, are susceptible to various bacterial and viral infections. Animals are used widely in biomedical research. Laboratory animal infections may compromise the health of the animals and ultimately the research data derived from them. Microbial infections alter not only the animal behavior but also the biological responses. Apart from the use of whole animals for experimentations, numerous animal cell lines and proteins are also derived from animals and used in biomedical research. So there is great potential for the diseases to spread very quickly.
Paramyxoviruses are viruses of the Paramyxoviridae family of the Mononegavirales order; they are negative-sense single-stranded RNA viruses responsible for a number of human and animal diseases. Virions are enveloped and can be spherical, filamentous or pleomorphic. Fusion proteins and attachment proteins appear as spikes on the virion surface. Matrix proteins inside the envelope stabilise virus structure. The nucleocapsid core is composed of the genomic RNA, nucleocapsid proteins, phosphoproteins and polymerase proteins. The gene sequence is:
A number of important human diseases are caused by paramyxoviruses. These include mumps, measles, and respiratory syncytial virus (RSV), which is the major cause of bronchiolitis and pneumonia in infants and children. Paramyxoviruses are also responsible for a range of diseases in other animal species, for example canine distemper virus (dogs), phocine distemper virus (seals), cetacean morbillivirus (dolphins and porpoises), Newcastle disease virus (birds), and rinderpest virus (cattle). Pneumonia virus of mice (PVM) is a member of the subfamily Pneumovirinae and is the closest known relative of respiratory syncytial virus. Both viruses cause pneumonia in their respective hosts. Serological evidence revealed that PVM is prevalent among many species of laboratory rodents, in which it causes a latent or inapparent infection. There is serologic evidence suggesting widespread exposure of humans to PVM. The degree of amino acid sequence identity between PVM and RSV ranges from 10% (M2-2 protein) to 60% (nucleocapsid N protein). The pathogenesis of PVM in inbred mice varies considerably between strains; in the commonly used BALB/c strain, the virus is highly pathogenic. There has been serologic evidence of infection of a number of other laboratory animals, including other rodent species, rabbits, and nonhuman primates.

Source:
Recombinant protein corresponding to 393aa from full-length mouse PVM-NP (PVM15 strain), fused to His-tag, expressed in E. coli.

Molecular Weight:
~44kD

Applications:
Suitable for use in ELISA, Western or other applications where native protein is required. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng antigen/well
Western Blot: load 100-200ng/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.