PLGF-1, Recombinant, Human (Placental Growth Factor­1, PGFL, PLGF)

Blue Ice

29, 7

Supplied as a lyophilized powder from 10mM sodium phosphate. No preservative added. Reconstitute with 250ul sterile ddH2O. For extended storage, the additions of 0.1% bovine serum albumin (BSA) is recommended.

Placental growth factor (PLGF) is a dimeric glycoprotein and member of the vascular endothelial growth factor (VEGF) family, involved in angiogenesis, and the stimulation of endothelial cell proliferation and migration. The three isoforms PLGF-1, PLGF-2 and PLGF-3 are present in most placental tissues and act through binding to the VEGFR-1/FLT1 receptor. PLGF has become a focus area in the study of angiogenesis and inflammatory diseases such as rheumatoid arthritis and psoriasis, and PLGF is used as a biochemical marker in cardiovascular disease, and also detected at reduced levels during preeclampsia.

Recombinant protein corresponding to human Placental Growth Factor expressed in E. coli

Molecular Weight:
~29.7kD

Amino Acid Sequence (Monomer):
MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR

Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 1-10ng/ml.

Storage and Stability:
Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.