PIM1, Active, Recombinant, Human (Oncogene PIM-1, Pim1 Kinase, Serine/threonine-protein Kinase Pim-1, PIM-1)

Dry Ice

62

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

The proto-oncogene Pim1 belongs to a family of serine/threonine protein kinases that are highly conserved through evolution in multicellular organisms. Originally identified from Moloney murine leukemia virus induced T-cell lymphomas in mice, Pim1 is involved in the control of cytokine-mediated cell proliferation, differentiation and survival of lymphoid and myeloid cells as well as others. Expression of Pim1 can be stimulated by a variety of growth factors and is regulated at four different levels: transcriptional, post-transcriptional, translational and post-translational. Accumulating data support that the expression of Pim1 is mediated through activation of the JAK/STAT pathway. Some of the substrates of Pim1 include p21 Cip1, nuclear mitotic appartus protein, PTP-U2S and Socs-1. Recently, Pim1 has been shown to enhance the activities of p100, c-Myb and Cdc 25a and in part this might explain reported effects of Pim1 on mitogenesis. Pim1 interacts with c-Myb via the DNA binding domain and regulates its transcriptional activity.

Source:
Recombinant protein corresponding to active human PIM1, expressed in Baculovirus (Sf9 insect cells) using a N-terminal GST tag.


Genebank:
NM_002648/PIM1

Molecular Weight:
~62kD

Concentration:
0.1ug/ul

Biological Activity:
~270nmol phosphate incorporated into the S6K substrate peptide/minute/mg protein at 30°C for 15 minutes using a final concentration of 50uM ATP (0.83uCi/assay).

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.