Anti-RANTES / CCL5 Antibody

ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting

Ambient temperature

Rabbit Anti-Human RANTES / CCL5

Phosphate buffered saline

Purified IgG - lyophilized

Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.

This product is specific for human RANTES (regulated upon activation, normal T cell expressed and secreted), otherwise known as CCL5, a 68 amino acid CC- chemokine involved in T cell activation and cell recruitment, expressed by cytotoxic (CD8+) T cells (CTLs), macrophages, keratinocytes and endothelial cells. RANTES acts as a chemoattractant for monocytes, CD4+/CD45RO+ memory T cells, basophils and eosinophils, thorough binding to chemokine (C-C motif) receptor 1 (CCR1), CCR3 and CCR5. Both CCR3 and CCR5 are known co-receptors for the entry of HIV viruses into target cells and the binding of RANTES to CCR3 on CTLs has been shown to enhance HIV-1-specific cytotoxicity, whilst binding to CCR5 inhibits membrane fusion and hence HIV entry.

Antisera to human RANTES were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.