Anti-TGF alpha Antibody

ELISA (dilution: 0.1ug/ml, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting

Ambient temperature

Rabbit Anti-Human TGF alpha

Phosphate buffered saline

Purified IgG - lyophilized

Reconstitute with 0.1 ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.

This product is specific for human TGF-alpha (transforming growth factor alpha), a 50 amino acid EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS). TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis.

Antisera to human TGF-alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was was prepared by antigen affinity chromatography.

*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.