Mouse anti-Histone [+Biotin]

Hersteller Raybiotech
Kategorie
Typ Antibody
Specific against Human
Isotype IgG1
Format Supplied as purified and lyophilized antibody in sterile PBS (pH 7.5), containing 0.1% BSA. The final concentration is indicated on the shipping vial. Please avoid freeze-thaw cycles.
Applikationen ELISA
Menge 50 ug
Host Mouse
ArtNr RB-14-0007B-50
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Target Name
Histone
Accession Number
Q71DI3
Gene Symbol
HIST2H3A
Gene ID
333932
Target Synonyms
histone 2, H3a, H3/n, H3/o
Conjugate/Tag/Label
Biotin
Application notes:
ELISA (recommended work dilution= 1:5000-8000 (Only recognized asymmetric form)), Western Blotting (recommended work dilution= 1-2 ug/ml)
Product Description
Histones are highly conserved and can be grouped into five major classes: H1/H5, H2A, H2B, H3, and H4. The tight packing of DNA into chromatin creates a need for mechanisms to relax chromatin and expose DNA for transcription, replication, and DNA repair. One of the mechanisms used by the cell to access DNA is the post-translational modification of Histone tails. Specifically, methylation of Histone tails generates a docking site for effector proteins, which aid in the recruitment of other enzymes necessary for the function at hand. Methylation at lysine and arginine residues within Histones has been linked to gene expression. In general, methylation of Histone residues lysine 4 and 36 on H3 are correlated with active gene regions, while methylation of lysine 9 and 27 on H3 is correlated with repressed gene regions. Very importantly, methylation of Histone residues arginie 2 on H3 (termed as H3R2) involved in gene expression.
Immunogen
Designed and synthetic peptide containing H3R2a was used as immunogen. This antibody was obtained from a mice immunized with the immunogen, then fusion, selection and identification. This antibody was produced from a hybridoma resulting from the fusion of a myeloma with B cells deriveded from the immunized mouse. The IgG fraction of cell culture supernatant was purified by Protein G affinity chromatography.

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Menge: 50 ug
Lieferbar: In stock
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