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Rat Notch-1 ELISA

Rat Notch-1 ELISA

Hersteller	Raybiotech
Kategorie	ELISA ELISA & Immunoassays ELISA Kits Immunoassay
Typ	Elisa-Kit
Specific against	Rat
Menge	5 Plate Kit
ArtNr	ELR-NOTCH1-5
eClass 6.1	32160605
eClass 9.0	32160605
Lieferbar
Species Detected	Rat
Compatible Sample Types	Cell Culture Supernatants Plasma Serum
Design Principle	Sandwich-based
Method of Detection	Colorimetric
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Kit Components	Pre- Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin- Conjugated H R P T M B One- Step Substrate
Other Materials Required	Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for E L I S A data analysis
Protocol Outline	Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at R T or O/ N at 4° C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at R T. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at R T. Add 100 µl of T M B One- Step Substrate Reagent to each well. Incubate 30 min at R T. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Protein Name & Synonyms	Neurogenic locus notch homolog protein 1 (Notch 1) [Cleaved into: Notch 1 extracellular truncation (NEX
Recommended Dilution (serum & plasma)	20 fold
Additional Notes	Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data

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