Recombinant Human Syntaxin-6 Protein, HEK293 Supernatant

Proteins|Recombinant Proteins|Human Proteins

Blue ice

Recombinant protein has a calculated molecular weight of about 27 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).

Not applicable

12352202

Ser2-Gln234

Pink liquid

Not determined

Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.