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Recombinant Human HSP-60 Protein, HEK293 Supernatant

ArtNr 230-20191-200
Hersteller Raybiotech
Menge 200 ul
Kategorie
Typ Proteins
Format Liquid
Applikationen WB, IP, ELISA, Dot
Specific against Human (Homo sapiens)
Host HEK293 cells
Konjugat/Tag HIS
Purity Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammali
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Applications
Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair test), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein functional assay, protein-protein interaction, post-translational modifications, etc.
Manufacturer - Category
Proteins|Recombinant Proteins|Human Proteins
Manufacturer - Conjugate / Tag
N-terminal histidine tag
Shipping Temperature
Blue ice
Storage Conditions
-20°C
Molecular Weight
Recombinant protein has a calculated molecular weight of about 58 kDa. The actual molecular weight may increase slightly due to the potential post-modifications (PTMs).
Description
Recombinant Human HSP-60 overexpression cell culture supernatant, secreted from the serum-free medium of transfected HEK293 cells (200 µl). Purchase will also include one vial of normal control, the culture supernatant of HEK293 cell transfected with empty expression vector.
Purity determined by
Not applicable
Gene Symbols
HSP60, HSPD1
UNSPSC Code
12352202
Formulation
Culture supernatant of transfected HEK293 cells
Expressed Region
Ala27-Phe573
Protein Name & Synonyms
60 kDa heat shock protein, mitochondrial (60 kDa chaperonin) (Chaperonin 60) (CPN60) (Heat shock protein 60) (HSP-60) (Hsp60) (HuCHA60) (Mitochondrial matrix protein P1) (P60 lymphocyte protein), 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, HuCHA60, Mitochondrial matrix protein P1, P60 lymphocyte protein, Heat Shock Protein Family D (Hsp60) Member 1, Heat Shock 60kDa Protein 1 (Chaperonin), Spastic Paraplegia 13 (Autosomal Dominant), Heat Shock 60kD Protein 1 (Chaperonin), Short Heat Shock Protein 60 Hsp60s1, Heat Shock Protein 65, GROEL, HSP65, SPG13, HLD4
Format
Pink liquid
Concentration
Not applicable
Endotoxin Level
Not determined
Preservative
None
Preparation
Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 °C immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 200 ul
Lieferbar: In stock
lieferbar

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