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Recombinant Human ERBB2 (Her2) (C-Terminal) Protein, HEK293 Lysate

ArtNr 230-10100-100
Hersteller Raybiotech
Menge 100 ug
Kategorie
Typ Proteins Recombinant
Format Lyophilized
Applikationen WB, IP, ELISA, Dot
Specific against Human (Homo sapiens)
Host HEK293 cells
Konjugat/Tag HIS
Purity Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Tyrosine kinase-type cell surface receptor HER2,Erb-B2 Receptor Tyrosine Kinase 2,HER2,NGL,NEU,V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2,Tyrosine Kinase-Type Cell Surface Receptor HER2,Neuro/Glioblastoma Derived Oncogene Homolog
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Applications
Western blotting control, antibody validation (i.e., hybridoma screening, antibody pair screening), ELISA, EIA, dot blotting, immunoprecipitation (IP), protein array, protein-protein interaction studies
Manufacturer - Category
Proteins|Recombinant Proteins|Human Proteins
Manufacturer - Conjugate / Tag
N-terminal 6x histidine tag
Shipping Temperature
Ambient temperature
Storage Conditions
-20°C
Molecular Weight
Recombinant human ERBB2 has a calculated molecular mass of 65 kDa. Due to the abundant glycosylation, it migrates as approximately 70-80 kDa protein bands in Western blotting under DTT, beta-mercaptoethanol reducing conditions.
Description
Recombinant human ERBB2, C-terminal (Receptor tyrosine-protein kinase erbB-2) overexpression cell lysate, derived from the transfected HEK293 cells. (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog # 230-10006) transfected with empty expression vector.
Gene Symbols
ERBB2, HER2
UNSPSC Code
12352202
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Expressed Region
Lys676-Val1255
Protein Name & Synonyms
Tyrosine kinase-type cell surface receptor HER2, Erb-B2 Receptor Tyrosine Kinase 2, HER2, NGL, NEU, V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2, Tyrosine Kinase-Type Cell Surface Receptor HER2, Neuro/Glioblastoma Derived Oncogene Homolog, Human Epidermal Growth Factor Receptor 2, Metastatic Lymph Node Gene 19 Protein, Proto-Oncogene C-ErbB-2, Proto-Oncogene Neu, P185erbB2, MLN 19, V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncogene Homolog 2 (Neuro/Glioblastoma Derived Oncogene Homolog), V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog, V-Erb-B2 Avian Erythroblastic Leukemia Viral Oncoprotein 2, Neuroblastoma/Glioblastoma Derived Oncogene Homolog, Receptor Tyrosine-Protein Kinase ErbB-2, C-Erb B2/Neu Protein, CD340 Antigen, HER-2/Neu, Herstatin, CD340, HER-2, MLN19, TKR1
Format
Lyophilized powder
Purification
Unpurified overexpression cell lysate
Concentration
Determined by BCA protein assay kit (Thermo Scientific)
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level
Not determined
Preservative
None

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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