Recombinant Mouse INTERLEUKIN-4

Hersteller Raybiotech
Kategorie
Typ Proteins
Specific against Mouse
Menge 20 ug
ArtNr 229-20306
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Short Description
Purified recombinant IL-4 expressed in E.coli
Introduction
Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, is a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes.
Protein Name
IL-4
Molecular Weight (kDa)
13.5kD (120 amino acid residues)
Purity
>98% by SDS PAGE and HPLC analysis
Concentration
ca.0.1mg/ml after reconstitution
Preservative
None present
Endotoxin Level
<1EU/ug
Protein Type
Recombinant Protein
Recommended Applications
ELISA (0.2-0.4ng/well), Functional Assays, Western Blotting (1.5-3.0ng/lane)
Biological activity
The ED50 was determined by the dose-dependent proliferation of murine HT-2 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.
Reconstitution
Reconstitute with 200ul distilled water.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommends that the vial is gently mixed after reconstitution.
Storage/stability
Prior to reconstitution store at +4° C. Following reconstitution store at -20° C.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. This protein may be stored for3 months from date of reconstitution.
Menge: 20 ug
Lieferbar: In stock
Listenpreis: 528,00 €
Preis: 528,00 €
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