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Recombinant Human ALK Protein

ArtNr 230-10003-100
Hersteller Raybiotech
Menge 100 ug
Kategorie
Typ Proteins Recombinant
Format Lyophilized
Applikationen WB, ELISA, other, Dot
Specific against Human (Homo sapiens)
Host Human, HeLa Cells
Konjugat/Tag HIS
Purity Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Applications
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc.
Manufacturer - Category
Proteins|Recombinant Proteins|Human Proteins
Manufacturer - Conjugate / Tag
C-terminal 6x histidine tag
Manufacturer - Host
Human HeLa Cell Line
Shipping Temperature
Ambient temperature
Storage Conditions
-20°C
Molecular Weight
115 kDa
Description
Recombinant human Anaplastic Lymphoma Kinase (ALK) Overexpression Mammalian Cell Lysate (100 µg). Purchase will also include one vial of normal control HEK293 cell lysate (Catalog # 230-10006) transfected with empty expression vector.
Gene Symbols
ALK
UNSPSC Code
12352202
Formulation
Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail
Expressed Region
Val19-Ser1038
Protein Name & Synonyms
Anaplastic lymphoma kinase, ALK tyrosine kinase receptor, CD246, anaplastic lymphoma receptor tyrosine kinase.
Format
Lyophilized powder
Purification
HeLa cells, tansfected with human ALK overexpression vector and empty vector respectively, are harvested and washed with PBS. The cells were further lyzed with Raybiotech Mammalian Cell Lysis Buffer (compatible with most enzyme assays) containing mammalian cell protease inhibitor cocktail. After clarified with 20, 000 g centrifugation for 30 min, the lysate protein concentration was determined by BCA kit (Thermo Scientific Inc.). The lysates were aliquoted and stored at , 80 , C immediately.
Concentration
Lot specific (see the label on the vial).
Reconstitution
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5~1 mg/mL with sterile deionized water.
Endotoxin Level
Not determined
Preservative
None

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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